17 March 2003
A mouse model for studying rapid intraoperative methods of skin closure and wound healing.
Xi Zhu, David Hall, Glenn Ridenour, Sohyun Boo, Tucker Jennings, Julio Hochberg, Eugene Cilento, Frank ReillyMed Sci Monit 2003; 9(3): BR109-115 :: ID: 4738
Abstract
BACKGROUND: We re-examine the adequacy of an albino (hairless) mouse dorsal distally based skin flap model by determining elastic modulus and by assessing viability after application of rapid intermittent stretch to facilitate wound closure. MATERIAL/METHODS: Modulus of elasticity was determined with materials testing. Viability was accessed by laser Doppler flowmetry and by quantifying marginal tissue necrosis (mm2) at 5 days. Effective length gained and tension relieved were measured by subjecting flaps to simple undermining plus 3-10 g of initial tension (linear stretch) using a skin hook. Immediate hemispherical stretch was applied with an implanted subcutaneous silicone balloon (Rapid Intraoperative Tissue Expansion or RITE) at 1 or 2 cycles of 3-8 min and 6-12 ml for each cycle. Flaps subjected to simple and extensive undermining with or without application of initial linear stretch served as postoperative controls. RESULTS: The elastic modulus of 9.85 (1.02) N/mm2 approximated the skin of the human face. Flowmetry demonstrated that all flaps were adequately perfused. Stretching significantly decreased flap tension and increased flap length over simple undermining alone. It drastically improved flap viability by provoking a 50-75% decline in 5-day necrosis. The benefits were dependent upon the initial linear tension applied, but independent of the timing, cycling, and volume selected for RITE and the flap dimensions at closure. CONCLUSIONS: Results demonstrate that the albino (hairless) mouse dorsal distally based skin flap is a reliable model for studying wound closure and that rapid intermittent stretching in this model facilitates closure and wound healing.
Keywords: Elasticity, Laser-Doppler Flowmetry, Mice, Inbred HRS, Models, Animal, Skin - blood supply, Skin - injuries, Skin - surgery, Stress, Mechanical, Surgical Flaps, Surgical Procedures, Operative, Wound Healing, Wounds and Injuries - surgery
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
05 Mar 2024 : Clinical Research
Effects of Thermal Insulation on Recovery and Comfort of Patients Undergoing Holmium Laser LithotripsyMed Sci Monit In Press; DOI: 10.12659/MSM.942836
05 Mar 2024 : Clinical Research
Role of Critical Shoulder Angle in Degenerative Type Rotator Cuff Tears: A Turkish Cohort StudyMed Sci Monit In Press; DOI: 10.12659/MSM.943703
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952